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Research Report A leading global R&D planning and evaluation institute for technological innovation in food, agriculture, forestry and fisheries

LAST UPDATE : 2016-01-01

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Subject Development of health functional foods for the improvement.....
Date 2017-02-01 16:11:18 Hit 340
Data
Contents Ⅰ. Title : Development of health functional foods for the improvement of blood circulation from
Ligularia fischeri extract

II. Achivements of Research Development and Implementation Performance

III. Objectives and necessity of the research
1. Research Objectives
The final goal of this research is to develop health-functional food for improving blood
circulation using Ligularia fischeri extracts and to establish a mass production and safety
assessment of functional ingredient. This research was performed to be acquired for
individual health-functional food ingredients by proving the scientific evidence and efficacy
evaluation of domestic agricultural resources. In addition, this research was intended for
industrialization of personalized health-functional food.
2. Necessity of the research
In modern society, metabolic syndrome accompanied with blood circulation disorder is
increasing due to westernization diet, industrialization and emotional sterss. Also, the
prevalence rate of metabolic syndrome increased dramatically over the past decade in
Korea. Therefore, establishment of the efficacy and safety of a variety of health food is
required in order to protect health of people and to strength national competitiveness. In
addition, because abdominal obesity and obese children are growing as social problems, the
development of health-functional food for improving blood circulation and lipid metabolism
by the domestic agricultural resources is required.

IV. The contents of the study
○ Identification of marker compounds for standardization of as functional ingredient
- Deternination and analysis of active components
- Optimization of extrraction process of functional ingredients
- Development of protocol for clinical trial
○ Establishment of standardized optimal extraction process
○ Pilot scale extraction with mass-up process
○ Isolation and analysis of bioactive components from optimal extracts
○ Standardizing of specifications for raw materials and validation of raw materials
○ Design of hμMan trial and approval of IRB
○ The evaluation of anti-oxidant effect using the Ligularia fischeri extract(LFE)
○ The evaluation of inhibitory effect of LFE and active compound (ethyl caffeate) on adipocyte
differentiation in preadipocytes
○ The evaluation of inhibitory effect on platelet agglutination and antithrombotic effect
using LFE and active compounds in vitro
○ In vivo evaluation test for improvement of blood circulation using LFE
○ Investigation of efficacy and functional maechanism of Ligularia fischeri extract for
improving blood circulation
○ Active components of Ligularia fischeri in regulating angiogenesis and cancer
progression

V. The results of Research and Development
○ Isolation of 12 compounds including marker and bioactive components. Establishment of
optimization analytical condition using HPLC for marker and bioactive components.
○ Establishment of optimization analytical condition using HPLC and standardizing of
specifications for raw materials according to HPLC method validation for raw materials
and marker compounds.
○ Pilot scale extraction with mass-up process using standardized optimal extraction
process.
○ The evaluation of anti-oxidant effect using the Ligularia fischeri extract (LFE)
We examined the tasting for free radical scavenging activity with ABTS and DPPH assay, to
confirm the anti-oxidant effect of LFE depending on region and dosage. As a result, we
showed the Inje regional sample (50% EtoH extract of Ligularia fischeri) has the strongest
anti-oxidant effect.
○ The evaluation of inhibitory effect of LFE and active compound (ethyl caffeate) on adipocyte
differentiation in preadipocytes
To measure the adipogenic effect of LFE and active compound, we examined the testing for
adipocyte differentiation. LFE and compounds treatment resulted in the dose-dependent
decrease of triglyceride accμMulation. Especially, ethyl caffeate suppressed the expression of
adipogenic marker genes and also inhibited the expression of PPARγ and CEBPα at both
mRNA and protein levels in preadipocytes.

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