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LAST UPDATE : 2016-01-01
|Subject||Development of a novel FMD vaccine with molecular immunoadjuvant|
Title of Project : Development of a novel FMD vaccine with molecular immunoadjuvant
It has been known that swine farmers in Korea have suffered from
nonsuppurative granuloma by the present FMD killed vaccine. Since the
commercial FMD vaccine is made of the killed FMD virus antigen loaded with
double shelled oil, the vaccine may have produced granuloma without pus on
vaccination site. One of ways of reduction of the granuloma is to not use
DOE, but unavoidably immune responses decrease. Therefore the aim of this
project was to develop vaccine with increasement of immune responses against
FMDV without granuloma either by use of vectored or/and recombinant vaccine
or/and with molecular immune-adjuvant.
One of strategies to develop vectored vaccine for the ideal FMD vaccine is to
use bacteria or virus. In the wake of well-known vaccine in anti-cancer
therapeutic tool, we constructed adenovirus-based vectored vaccine up-loaded
with FMDV P12A3C genes which produce structural polyproteins including VP1,
VP2, VP3, and VP4. The other strategy is to use known molecular adjuvant.
Among many types of molecular adjuvants, poly(I:C), interferon-α, and
GM-CSF were included for the purpose of this project.
Adenovirus constructed with FMDV genes VP1, VP2, VP3, and VP4 was
produced as much as 107.5-8TCID50/ml and concentrated into 5 x 1010TCID/dose
using TFF filter system and then injected on ear muscle of SPF pigs.
Protective neutralizing antibody titers more than 16 fold were formed compared
to control. When the FMD vector vaccine was inoculated into commercial
growing pigs which had maternal antibody. Titers of neutralizing antibody were
increased to the 16-fold level to which pigs would be protected from challenge.
In another experiment, when PERV-GM CSF was constructed and inoculated
along with commercial FMD vaccine into mice, immune responses were
elicited and some reached the level of protection.
It is concluded that the O type FMDV adenovirus vector vaccine may be useful
to protect pigs from FMD and GM-CSF in PERV system may be useful as
killed vaccine adjuvant.